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²Î¿¼ÎÄÏ×:
1.Arlotta, P, (2018). Organoids required! A new path to understanding human brain development and disease.Nature methods.15(1).27-29.

2. Gu, 0.. Tomaskovic-Crook, E., Wallace, G. G., & Crook, ]. M. (2018). Engineering Human Neural Tissue by 3D Bioprinting. In Biomaterials for Tissue Engineering (pp. 129-138). Humana Press, New York, NY.

3. Kang, P, Kumar, S., & Schaffer, D. (2017). Novel biomaterials to study neural stem cell mechanobiologyand improve cell-replacement therapies, Current opinion in biomedical engineering, 4, 13-20.

4. Tang, Y., Yu, P, & Cheng, L. (2017). Current progress in the derivation and therapeutic application of neural stem cells.Cell death & disease, 8(10), e3108.

5.Kelava, l., & Lancaster, M, A. (2016). Stem cell models of human brain development, Cell stem cell 18(6),736-748.

6.Gage, F, H., & Temple, S. (2013). Neural stem cells: generating and regenerating the brain. Neuron,80(3).588-601.

7.Zhuang, P., Sun, A, X., An, j., Chua, C, K., & Chew, S. Y, (2018). 3D neural tissue models: From7. spheroids to bioprinting.Biomaterials,154,113-133.